Objectives of Study:
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Polycystic Ovary Syndrome (PCOS) is the most common endocrine and metabolic disorder in women of reproductive age, with an incidence of 5%-10% in women of reproductive age. The pathological characteristics of PCOS Ovary are ovulation dysfunction and abnormal follicular development, which are mainly manifested as: Preantral and antral follicles increase and lack of dominant follicle development results in anovulation dysfunction. The clinical features of the syndrome are hyperandrogenemia and persistent anovulation, with multiple small 3-10mm follicles in both ovaries and no dominant follicles. It is often associated with metabolic problems such as obesity, insulin resistance and dyslipidemia, and is prone to endometrial cancer, type 2 diabetes and cardiovascular and cerebrovascular diseases, seriously affecting women's reproductive health and quality of life. IVF treatment for PCOS patients has been widely reported. Although PCOS patients can produce a large number of oocytes through ovulation induction therapy, these oocytes are usually of poor quality, followed by a low rate of fertilization, cleavage and implantation of embryos, and a high rate of abortion. At present, the etiology and pathological mechanism of PCOS are not completely clear. It is suggested that the etiology and pathological mechanism of PCOS are closely related to apoptosis regulation. Impaired ovum maturation and reduced embryonic development potential in PCOS women may be related to abnormal endocrine/parafontal factors, abnormal metabolism and changes in follicular environment during follicular development and maturation. Therefore, a deeper understanding of the relationship between PCOS and abnormal internal and external ovarian factors, as well as its influence on granulosa cell-oocyte connection, oocyte maturation and embryo development potential, is crucial for improving fertility of patients, optimizing clinical ovarian stimulation programs and improving pregnancy rate.
Heat shock protein 70(HSP70) is one of the most sensitive stress protective proteins widely existing in eukaryotic and prokaryotic organisms. It has molecular chaperone function, anti-radiation function, and enhances the anti-stress, anti-apoptosis and anti-oxidation function of cells, as well as promotes cell proliferation and participates in immune regulation. HSP70 acts as an anti-apoptotic protein to protect cells from TNF, monocytes, oxidative stress, chemicals, ceramides, and radiation-induced cytotoxicity. Nuclear transcription factor KappaB(NF-κB) is an important transcription factor at the intersection of multiple signal transduction pathways in eukaryotic cells, involved in various gene regulation, immune response and apoptosis signal transduction processes. It was inactive in cytoplasm in combination with inhibitory factor IκBα and NF-кB showed bidirectional regulation on apoptosis, which could both promote and inhibit apoptosis. The aim of this study was to verify the difference of HSP70/NF-κB/Caspase3 expression in granulosa cells of PCOS patients. To explore the clinical efficacy and mechanism of Cangfu Daotan Prescription granules in the treatment of phlegm-dampness PCOS patients. To observe whether Cangfu Daotan Fang granules regulate HSP70/NF-κB/ Caspase3 signaling pathway to inhibit granulosa cell apoptosis and improve ovarian function, in order to provide theoretical basis and experimental support for clinical targeted treatment of PCOS.
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Exclusion criteria:
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① Those who do not follow the doctor's advice or are allergic to the Chinese medicine ingredients
② Complicated with endometriosis, adenomyosis, ovarian tumor, decreased ovarian reserve function, pelvic inflammatory disease and other systemic diseases affecting ovarian function
③ Infertility caused by genetic factors
(4) a history of recurrent abortion
⑤ Abnormal anatomical structure of female reproductive tract does not have pregnancy function
⑥ The woman suffers from serious physical or mental disease and cannot bear pregnancy
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